What is the principle of Papanicolaou stain?
The principle of pap stain is to clearly distinguish between basophilic and acidophilic cell components and obtain a detailed chromatin pattern. The pap stain has 3 solutions having 6 dyes.
What is the significance of the Papanicolaou test in diagnostic cytology?
Detection of HPV-Associated Lesions Papanicolaou tests (also known as Pap tests) are named after their inventor, Georgios Papanicolaou, and serve to detect HPV-associated lesions in the cervix.
What is the meaning of Papanicolaou?
Listen to pronunciation. (PA-puh-NIH-koh-low…) A procedure in which a small brush is used to gently remove cells from the surface of the cervix and the area around it so they can be checked under a microscope for cervical cancer or cell changes that may lead to cervical cancer.
Who invented Papanicolaou?
George Nicholas Papanicolaou
George Nicholas Papanicolaou was a pioneer in elucidating the physiology and cytologic characteristics of the female reproductive system. He is best known for creating the Papanicolaou test, commonly known as the Pap smear, which revolutionised the early detection of cervical cancer.
How are you tested for HPV?
What you can expect. An HPV test is usually done at the same time as a Pap test — a test that collects cells from your cervix to check for abnormalities or the presence of cancer. An HPV test can be done using the same sample from the Pap test or by collecting a second sample from the cervical canal.
Why is it called Papanicolaou?
It is named after Georgios Papanikolaou, the doctor who determined its use in detecting early signs that could lead to cervical cancer. It is one form of cervical cancer screening.
What is Parakeratosis cervix?
In the cervix, parakeratosis/hyperkeratosis is known to be associated with uterine prolapse, chronic irritation and inflammation, diaphragm or pessary use, and posttherapy. 9 It grossly presents as focal or diffuse thickened white plaque; when visualised under colposcopy, it will generally be subjected to biopsy.
PRINCIPLE OF PAPANICOLAOU STAIN. Papanicolaou stain includes both acidic and basic dyes. Acidic dye stains the basic components of the cell and basic dye stain the acidic components of the cell. The polychromatic PAP stain involves five dyes in three solutions.
When was the Papanicolaou technique developed?
The technique was developed by George Papanicolaou in 1942. To define the cell nuclear to aid in the identification of nuclear abnormalities of cancer cells. To differentiate and identify certain cell types such as acidophils and basophils.
Can the modified Ultrafast Papanicolaou stain be used for fine needle aspiration cytology?
The aim of the study was to assess the use of the modified ultrafast Papanicolaou (MUFP) stain for fine needle aspiration cytology (FNAC) of head and neck swellings in comparison with the routine PAP stain, hematoxylin and eosin (H and E), and Giemsa. Materials and Methods: Forty FNACs of head and neck swellings were collected.
What is the history of Pap staining?
Papanicolaou (PAP) staining was first described by Papanicolaou in 1943 and widely used as a screening test despite being time consuming and requiring a large amount of alcohol.[5]