What kind of cell line is erythroleukemia cells?
K562 cells were the first human immortalised myelogenous leukemia cell line to be established. K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis.
What are murine erythroleukemia cells?
Murine erythroleukemia (MEL) cell lines are erythroid progenitor cells derived from the spleens of susceptible mice infected with the Friend virus complex 1. These virally transformed cells are arrested at the proerythroblast stage of development and can be maintained in tissue culture indefinitely (2).
What causes erythroleukemia?
History. At presentation, the signs and symptoms of erythroleukemia are usually nonspecific and are attributable to the decreased hematopoiesis resulting from the replacement of bone marrow by leukemic cells. This decrease results in anemia, thrombocytopenia, and leukopenia.
What is a research cell line?
A cell line is a collection of cells originating from one cell. Cell lines are typically kept in a growth medium in tubes, flasks, or dishes – called in vitro cell culture. Here, they can continue to divide indefinitely.
What are lymphoblastoid cells?
Lymphoblastoids. LCLs come from human lymphocytes. They are obtained by infecting peripheral blood lymphocytes (PBLs) with the Epstein–Barr virus (EBV). EBV immortalizes human B cells in vitro, enabling them to proliferate with an average population doubling time of 24 h (Nilsson et al., 1971).
Why are K562 cells used?
In human NK cell assays, the leukemic K562 cells are commonly used as targets for their high and reproducible sensitivity to NK lysis. The K562 cell line was originally considered as a highly undifferenciated cell line of the granulocytic lineage (Lozzio and Lozzio, 1975).
What is acute Erythroleukemia?
Erythroleukemia is a subtype of acute myeloid leukemia (AML) that is distinguished by erythroblastic proliferation. Patients usuallly present with nonspecific signs and symptoms from the anemia, thrombocytopenia, and leukopenia resulting from the replacement of bone marrow by leukemic cells (see Presentation).
What is the purpose of a cell line?
Cell lines have revolutionized scientific research and are being used in vaccine production, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.
What are lymphoblastoid cell lines used for?
Abstract: Lymphoblastoid cell lines (LCLs) represent a convenient research tool for expanding the amount of biologic material available from an individual. LCLs are commonly used as reference materials, most notably from the Genome in a Bottle Consortium.
What is new in erythroleukemia cell lines?
A new human erythroleukemia cell line has been established. This line, designated HEL, is capable of spontaneous and induced globin synthesis, producing mainly G gamma and A gamma chains. Embryonic chains (epsilon, zeta) and alpha chains are detectable in very small amounts; beta chains are undetectable.
Is the K562 a human erythroleukemic line?
As glycophorin is exclusively found on erythroid cells in human bone marrow we conclude that the K562 is a human erythroleukemic line. Antigens, Surface / analysis
How does 12-o-tetradecanoyl-phorbol-13-acetate affect human erythroleukemia cells?
We investigated the effect of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the human erythroleukemia cell line, HEL, and found that TPA addition (10 (-6)-10 (-8) M) to HEL cell cultures induces morphological, functional, and biochemical changes in HEL cells that are characteristic for macrophage-like cells.
Where does globin RNA synthesis occur in erythroleukemia?
There is evidence that RNA-dependent globin RNA synthesis occurs in the cytoplasm of mouse erythroleukemia cells, and antisense transcripts were also found in erythroid spleen cells and reticulocytes from anemic mice (Volloch, 1986; Volloch et al., 1987 ).