What is the purpose of blue dextran?

Bearing Cibacron Blue F3G-A, blue dextran is used in affinity chromatography and protein chromatography allowing for the identification of proteins (purified or in crude cellular extracts), and improving the purification procedures for various proteins (1), as well as in gel chromatography as molecular weight markers …

Why is blue dextran used in molecular exclusion chromatography?

Blue dextran is also used for checking the column packing. A symmetrical peak of elution indicates homogeneity of packing. The inner volume (Vi) of the column can be determined by subtracting the void volume from the elution volume of small molecules such as glucose or tyrosine having K d — 1.0.

What wavelength does blue dextran absorb?

The elution of Blue Dextran can be conveniently monitored using absorbance at wavelengths of 254, 280 or 620 nm.

What is the molecular weight of blue dextran?

∼2,000 kDa
The Vo of a given column is based on the volume of effluent required for the elution of a large molecule such as blue dextran (molecular mass of ∼2,000 kDa, Catalog Number D4772).

Is dextran an anticoagulant?

Abstract. Carboxymethyl dextran benzylamide sulfonate/sulfates (CMDBS) are synthetic polysaccharides with anticoagulant activity.

What type of drug is dextran?

High-molecular weight dextran is a plasma volume expander made from natural sources of sugar (glucose). It works by restoring blood plasma lost through severe bleeding. Severe blood loss can decrease oxygen levels and can lead to organ failure, brain damage, coma, and possibly death.

What is void volume in chromatography?

Void volume refers specifically to the volume of the liquid phase contained inside a column. The same term is sometimes also used informally to refer to the volume of a cavity in the column/tubing or fittings. Void volume is also known as dead volume.

Is dextran a protein?

Dextran is a complex polysaccharide derived from bacteria and it consists of glucose subunits. Dextran, not only seems to possess antithrombotic properties, but it also forms scaffolds that are resistant to protein and cell adhesion.

What is the void volume?

Why is blue dextran used in gel filtration?

Blue dextran: It is too large and can’t get into the beads and therefore excluded from the gel (Kd=0), and pass the column in the void volume (space between gel beads). Blue dextran is often used as a marker to measured void volume.

What is the molecular weight of ovalbumin?

around 45 kDa
Chicken ovalbumin consists of 385 amino acids and has a molecular mass of around 45 kDa.