What is the unit for Vmax and Km?

Vmax is the maximum enzyme velocity in the same units as Y. It is the velocity of the enzyme extrapolated to very high concentrations of substrate, so its value is almost always higher than any velocity measured in your experiment. Km is the Michaelis-Menten constant, in the same units as X.

What are the units of kcat?

The units of kcat are moles of product/sec divided by moles of enzyme. In consequence, kcat resulted in 1/time units. Enzyme concentration can be M, mM, uM, etc. The result of kcat*[E] is rate units: mM/min, uM/s, etc.

Why is Hanes Woolf more accurate than Lineweaver Burk?

Hanes-Woolf Plot Just like the Lineweaver-Burk equation, the Hanes-Woolf equation is of the form y = mx + c. The Hanes-Woolf plot is thought to be more accurate than Lineweaver-Burk for the determination of kinetic parameters.

What units are km?

kilometre (km), also spelled kilometer, unit of length equal to 1,000 metres and the equivalent of 0.6214 mile (see metric system).

What are units of Vmax?

The Vmax unit is moles/min, moles/sec, µmoles/min, or µmoles/sec. Vmax depends upon the amount or the concentration of the enzyme as well as the structure of the enzyme. Biology definition: Vmax is the maximum initial velocity or rate of a reaction.

What is the unit of substrate concentration?

Substrate concentration is the amount of substrate present that can be turned into product and is most commonly measured in molarity (moles per liter). The concentration of substrates is often used to measure enzyme activity, which is based on the rate of a reaction (product formed over time).

What are Vmax units?

The Vmax unit is moles/min, moles/sec, µmoles/min, or µmoles/sec. Vmax depends upon the amount or the concentration of the enzyme as well as the structure of the enzyme.

What is the Hanes-Woolf plot used for?

Like other techniques that linearize the Michaelis-Menten equation , the Hanes-Woolf plot was used historically for rapid determination of the important kinetic parameters “Km”, “Vmax” and “Vmax/Km”, but it has been superseded by nonlinear regression methods that are significantly more accurate and no longer …