What are nestin-Cre mice?

These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous and homozygous for the transgenic insert are viable and fertile. Nestin-cre mice exhibit mild hypopituitarism, growth retardation, impaired fear response, and a metabolic phenotype.

What is a Cre transgenic mouse?

A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).

What is a Cre promotor?

First, Cre-driver strain is generated in which Cre recombinase is expressed by a promoter that specifically targets the cell or tissue of interest. Second, loxP flanked (floxed) DNA containing mouse strain is needed to be generated.

What is the purpose of Cre?

The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.

What is a Cre activity?

Cre recombinase-mediated DNA recombination is an established method for conditional control of gene expression in animal models. Regulation of its activity has been accomplished to impart spatial and/or temporal control over recombination of the target gene.

Can nestin-CRE transgenic mice be used to direct recombination to neural stem cells?

Nestin-cre transgenic mice have been widely used to direct recombination to neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs). Here we report that a readily utilized, and the only commercially available, Nestin-cre line is insufficient for directing recombination in early embr …

Can nestin-Cre mice be used for behavioral phenotyping of psychiatric disorders?

Behavioral phenotyping of Nestin-Cre mice: implications for genetic mouse models of psychiatric disorders Genetic mouse models based on the Cre-loxP system have been extensively used to explore the influence of specific gene deletions on different aspects of behavioral neurobiology.

Is nestin-CRE enough for recombination in early embryos?

Here we report that a readily utilized, and the only commercially available, Nestin-cre line is insufficient for directing recombination in early embryonic NSCs and NPCs.

What is the function of the nestin-CRE transgene?

Specifically, the Nestin-Cre/ERT2 transgene directs cre expression in the nestin-expressing cells in the brain, which are generally restricted to the subventricular zone (SVZ) and subgranular zone (SGZ).