What is cleaved PARP?

Cleaved PARP, which is a indicator of apoptosis, was detected in HEK293 cells transiently expressing gene X which has been known as a inducer of apoptosis when everexpressed.

How is PARP activated?

PARP-1 and PARP-2 are activated by DNA single-strand breaks, and both PARP-1 and PARP-2 knockout mice have severe deficiencies in DNA repair, and increased sensitivity to alkylating agents or ionizing radiation.

What is the difference between caspase-3 and cleaved caspase-3?

One specific effector caspase is caspase-3, a protein that is cleaved and thus activated upon the initiation of apoptosis. Cleaved caspase-3 propagates an apoptotic signal through enzymatic activity on downstream targets, including poly ADP ribose polymerase (PARP) and other substrates (2).

Why GAPDH is used as control?

GAPDH antibody, is usually used as a loading control antibody for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. OriGene offers high quality of Anti-GAPDH TrueRAB™ antibody (Loading control).

How many PARP are there?

17 PARPs
Poly(ADP-ribose) polymerases (PARPs) are a family of enzymes that catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) onto protein substrates, including PARPs themselves (i.e., automodification). There are 17 PARPs in humans, all sharing a domain of ∼230 amino acids.

How is the PARP antibody generated?

The PARP Antibody was generated using PPOL, poly (ADP-ribose) polymerase family, member 1, PARP-1, and PARP1 as the antigen. PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.

What is the role of PARP in cell division and apoptosis?

PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.

Is the PARP (46d11) antibody specific to HEK293 cells?

The absence of signal in the PARP knockout HEK293 cells confirms the specificity of the antibody for PARP. Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.

What is the sensitivity of rabbit antibody to PARP?

Specificity / Sensitivity. PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 and PARP-3.