What is HPTLC method?
High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements.
Which steps involved in HPTLC technique?
The HPTLC suite is composed of a number of components.
- Sample Applicator – Application of sample to stationary phase i.e. TLC/HPTLC plate.
- Development Chamber – Separation of components.
- Derivatizer set and Plate heater – Derivatization: Chemical spraying of plate to detect compounds not visible under white/UV light.
Is HPTLC quantitative?
Moreover, HPTLC has proven its value as a reliable quantitative analysis technique for the quality control of raw materials and products.
What is method validation in HPLC?
Validation is a formal and systematic way to demonstrate the suitability of the assay method to provide useful data to ensure that the process or the technique gives satisfactory and consistent results within the scope of the process. The analytical techniques refer to the way of performing the analysis.
Why is a HPTLC performed?
High-performance thin-layer chromatography (HPTLC) is employed widely in pharmaceutical industries for process development, identification, and detection of adulterants in herbal products; identification of pesticide content and mycotoxins; and in quality control of herbs and health food.
What is the difference between TLC and HPTLC?
TLC and HPTLC are two chemical techniques used to separate non-volatile components in a mixture. The key difference between TLC and HPTLC is that the absorbent material in TLC plate has large particles whereas HPTLC plates have very small particles of adsorbent material. TLC is thin layer chromatography.
What are the advantages of HPTLC?
HPTLC is highly efficient because of several reasons: short run time per sample (e.g. a total analysis time of 30 min for 15 samples means a run time of 2 min/sample), low solvent consumption per sample and no cross-contamination due to single use of the plate.
What are the applications of HPTLC?
Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern.
What is LoD and LoQ in HPLC?
LoD and LoQ are important calculations in HPLC. LoD stands for limit of detection while LoQ stands for limit of quantitation.
Which detector is used in HPTLC?
The CAMAG TLC Scanner 4 (densitometer) is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects. The chromatogram is evaluated under white or UV light.
What is the HPTLC method for paliperidone determination?
A new, economic, environmentally friendly and rapid high-performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of paliperidone. The HPTLC separation was achieved on an aluminium-backed layer of silica gel 60F 254 using methanol – ethyl acetate (8.0 + 2.0 v/v) as mobile phase.
What is the HPTLC method for PPD identification?
A new HPTLC method has been developed for the identification and quantification of PPD. Low cost of ingredients, faster speed, and satisfactory precision and accuracy are the main features of this method. Method was successfully validated as per ICH guidelines and statistical analysis proves that method is sensitive, specific, and repeatable.
What are the advantages of the new HPTLC method?
HPTLC also facilitates repeated detection of chromatogram with the same or different parameters.
What does HPTLC stand for?
First published on 12th February 2010 A new, economic, environmentally friendly and rapid high-performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of paliperidone.