What are L6 myotubes?
Immortalized rat skeletal (L6) myoblast cell line that was selected for high fusion potential and endogenous expression of GLUT4 in the myotube stage. Highlights: Differentiate with high reliability into a myotube muscle cell phenotype that naturally expresses the GLUT4 glucose transporter protein.
Why L6 myotubes are chosen for this assay?
When stimulated by insulin, L6 myotubes appeared to exhibit glucose uptake to a larger extent than C2C12 cells (Sarabia et al. 1990), suggesting that at present L6 myotubes could be the most promising candidate as an efficient in vitro model system to investigate glucose uptake in muscle cells.
What are C2C12 myotubes?
C2C12 myotubes express contractile proteins and, when left to differentiate for an extended period of time, can spontaneously contract. These properties make C2C12 cells an invaluable tool for understanding the molecular biology of muscle development. However, these cells do not perfectly mimic in vivo muscle fibers.
What is C2C12 cell line?
The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977. Developed for in vitro studies of myoblasts isolated from the complex interactions of in vivo conditions, C2C12 cells are useful in biomedical research.
What is glucose uptake assay?
The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). Simple, homogeneous protocol: After addition of 2DG, there are no wash steps—all steps are additions.
How big are C2C12 cells?
At the time of the biophysical studies, mean myotube diameter was 12 microns (range 5-25 microns), and mean length was 290 microns (range 130-520 microns).
Where are myotubes found?
Ontogenesis of Striated Muscle Irregular tubules show branching as the myotube matures, and these tubules are found in regions of cytoplasm containing myofilaments and forming sarcomeres.
What is a myotubes?
[ mī′ə-tōōb′ ] Save This Word! n. A developing skeletal muscle fiber with a tubular appearance.
What is a Myofiber?
Muscle tissue is a highly specialized type of tissue, made up of cells that have as their fundamental properties excitability and contractility. The cellular elements that make up this type of tissue are called muscle fibers, or myofibers, because of the elongated shape they have.
How do you grow L6 myocytes in α-MEM?
Briefly, L6 myocytes were grown in α-MEM with 10% FBS at 37°C under 5% CO2. Differentiation to L6 myotubes was performed by 2% HS containing medium in 48-well culture plates. The various amounts of samples were incubated with the cells for 24 h. Insulin and metformin were used as positive controls.
What is an L6/40?
The L6/40 was a light tank used by the Italian army from 1940 through World War II. It was designed by Ansaldo as an export product, and was adopted by the Italian Army when officials learned of the design and expressed interest.
What was the most successful L6 variant?
The most successful of the L6 variants was the Semovente 47/32, which eliminated the turret and substituted a 47 mm antitank gun in the open-topped hull. A final version late in the war was an ammunition carrier armed only with a single 8 mm Breda machine gun.
What is Myf-6 mutations?
Myf-6 (also known as MRF4 or Herculin) is important to myotube differentiation and is specific to skeletal muscle. Mutations in Myf-6 can provoke disorders including centronuclear myopathy and Becker muscular dystrophy. In specific muscle formation, mutations in associated genetic factors begin to affect specific muscular regions.