Is the Kozak sequence in the 5 UTR?

The ability of the Kozak sequence to start translation can result in novel initiation codons in the typically untranslated region of the 5′ (5′ UTR) end of the mRNA transcript.

Table of Contents

Where is the Kozak sequence located?

A feature specific to eukaryotic mRNA is the Kozak sequence [6], which extends from approximately position −6 to position +6, where +1 is assigned to the adenine of the START codon (throughout the present paper, all positions are given respective to the START codon).

Do I need a Kozak sequence?

The kozak sequence in mammalian mRNA acts as a enhancer for translation. it is required but not necessary.

Are all eukaryotic genes Colinear?

Are all eukaryotic genes colinear? No, because some eukaryotic genes have introns that are not translated.

What is the Kozak sequence and how to use it?

The Kozak sequence (A/GCC AUG G) enhances translation from the correct initiation codon. I suggest to include the sequence in your cloning primer.

Is the Kozak sequence required for a CMV clone?

The Kozak sequence (A/GCC AUG G) enhances translation from the correct initiation codon. I suggest to include the sequence in your cloning primer. The kozak sequence in mammalian mRNA acts as a enhancer for translation. it is required but not necessary. As said by Paolo, the CMV promoter will drive a strong expression.

What if I can’t clone the Kozak sequence upstream to ATG?

If for some reason you cannot clone this sequence upstream to your ATG you could try to express your protein anyway. The Kozak sequence (A/GCC AUG G) enhances translation from the correct initiation codon. I suggest to include the sequence in your cloning primer.

How many 8-mer promoter sequences are in the human genome?

We have determined the distribution of each of the 65,536 DNA sequences that are eight bases long (8-mer) in a set of 13,010 human genomic promoter sequences aligned relative to the putative transcription start site (TSS). A limited number of 8-mers have peaks in their distribution (cluster), and most cluster within 100 bp of the TSS.