What are high throughput screening assays?
High-throughput screening (HTS) is a drug discovery process that allows automated testing of large numbers of chemical and/or biological compounds for a specific biological target, for example through binding assays.
How do you perform high throughput screening?
High-throughput Screening Steps
- Consultation. Potential users are invited to discuss a screening idea with any member of the SMDC HTS group.
- Assay development. The assay is developed by the initiating lab, usually in their laboratory.
- Dry-run.
- Pilot screen.
- HTS.
- Data analysis.
- Rescreen.
- Preliminary SAR.
What are high throughput methods?
Definition. High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level.
What is high throughput flow cytometry?
For example, high-throughput flow cytometry is an ideal tool for cell-based applications involving screening suspension cells where multiple readouts are desired. A technique such as high-content microscopy might be utilized in assays using adherent cells where spatial or subcellular information is required.
What is high-throughput qPCR?
Real-time quantitative PCR (qPCR) is a preferred platform for high-throughput gene expression profiling, where large numbers of samples are characterized for hundreds of expression markers.
Why is high-throughput screening necessary?
The main goal of the HTS technique is to accelerate drug discovery by screening large compound libraries at a rate that may exceed a few thousand compounds per day or per week. It is of vital importance, because parallel and combinatorial chemical synthesis generates a vast number of novel compounds.
What is high-throughput virtual screening?
HTVS may be defined as a process of target validation which involves the search for bioactive molecules within a database of interest that match a given query structure.
What is an example of high-throughput technology?
Other technologies that enable high-throughput include automation, robotics, and liquid-handling robotics.
How does high throughput sequencing work?
While sequencing information has traditionally been elucidated using a low throughput technique called Sanger sequencing, high throughput sequencing (HTS) technologies are capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time.
What is microfluidic qPCR?
Microfluidic qPCR Enables High Throughput Quantification of Microbial Functional Genes but Requires Strict Curation of Primers.
What is the difference between high throughput screening and virtual screening?
Virtual screening, similar to HTS, relies upon assessing a large, diverse library of compounds. The difference is that the compounds in a virtual screening library are contained in databases rather than being physically assembled by the organisation conducting the screen.