What is a tetramer antibody?

A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within a blood sample.

Table of Contents

What is ova tetramer?

OVA Tetramer – SIINFEKL Tetramer Ovalbumin (OVA) is a T Cell-dependent antigen that is frequently used as a model protein for studying antigen-specific immune responses in ovalbumin sensitized C57BL/6 mice or in TCR transgenic OT-I mice.

How do you make a MHC tetramer?

MHC tetramers are prepared by mixing enzymatically biotinylated MHC molecules with commercial preparations of streptavidin, usually conjugated to a fluorescent phycobiliprotein such as phycoerythrin (PE) or allophycocyanin (APC).

What is Siinfekl peptide?

Ovalbumin peptide SIINFEKL (H-2 Kb) is a single peptide for stimulation of T cells. The peptide from Ovalbumin is synthesized as it is presented by the MHC class I H-2 Kb allele and can be used for targeted in vitro generation and expansion of antigen-specific CD8+ T cells.

How does a tetramer work?

Tetramer analysis is a technique that uses MHC molecules to detect T-cells that recognize peptides or antigens from infected cells or cancerous cells. A tetramer has four MHC molecules attached to a streptavidin-coated bead. T-cells that recognize the peptide in the four MHC molecules will bind to the tetramer.

Can you fix tetramer stained cells?

Fixation of cells is compatible with Tetramer staining but only when fixing samples after Tetramer staining. Do not attempt to fix samples prior to Tetramer staining. Fix cells for 1-4 hours at r.t. in 1% methanol-free formalin in PBS following staining and washing. Commercial fixing kits can also be used.

What is h2kb?

Abstract. The peptide-binding site of the murine MHC class I molecule H-2Kb contains a deep C pocket, that is critical for peptide binding, as it accepts the anchor phenylalanine or tyrosine residue located in the middle (position 5, P5F/Y) of H-2Kb binding peptides. H-2Kb predominantly binds octameric peptides.

What are tetramers used for?

A tetramer assay (tetramer stain) is used to detect and quantify antigen specific T cells. The interaction between a peptide MHC complex (pMHC) and a cognate T cell receptor (TcR) is of too low affinity to be detected in flow cytometry.

Why is Haemoglobin a tetramer?

Hemoglobin is a tetrameric protein that binds and transports four oxygen molecules per unit and then releases them to myoglobin. The binding of oxygen to hemoglobin is allosterically cooperative, in that the binding of each oxygen molecule facilitates the binding of the next.

Where does ovalbumin come from?

Ovalbumin is the major protein component of egg white, which represents 45% of the total proteins. As eggs age, or upon thermal treatment such as pasteurization of egg white, native ovalbumin is converted into a de-aminated form, known as S-ovalbumin.

What is the purpose of a tetramer assay?

Do tetramers activate T cells?

CD8-null, wild-type and CD8-enhanced peptide–major histocompatibility complex class I (pMHCI) tetramers enable the staining of T cells with increasingly weak T-cell receptor (TCR)/pMHCI interactions.

What is pMHC tetramer made of?

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of OVA-derived peptide of SIINFEKL sequence covering 257-264 and H-2Kb molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

Can the pMHC tetramer be used for antigen-specific T cells?

The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells. To download a Certificate of Analysis, please enter a lot number in the search box below.

How do I get a certificate of analysis for the pMHC tetramer?