What is the matK gene?
Maturase K (matK) is a plant plastidial gene. The protein it encodes is an organelle intron maturase, a protein that splices Group II introns. It is essential for in vivo splicing of Group II introns.
What is the function of matK?
MatK is proposed to aid excision of seven different chloroplast group IIA introns that reside within precursor RNAs for essential elements of chloroplast function. We have developed an in vitro activity assay to test chloroplast group IIA intron excision.
What are rbcL and matK used for?
The joint use of DNA barcoding markers rbcL and matK is a reliable tool for identification of land plants from Sumatra, Indonesia and the addition of further markers, such as the trnL-F marker, provide better node support of the lineages.
What is rbcL gene?
The rbcL gene is the barcode DNA for plant species. Ternate Island is one of clove plantation center in North Maluku. The diversity and productivity of cloves on the Ternate island known since in earlier times.
Where is matK gene found?
chloroplast
The matK gene of chloroplast is 1500 bp long, located within the intron of the trnK and codes for maturase like protein, which is involved in Group II intron splicing.
Where is matK found?
chloroplast genomes
The matK reading frame is found in all known autotrophic land-plant chloroplast genomes that contain group II introns, and is also present in basal streptophyte algae (Turmel et al., 2006).
How many amino acids are in matK?
sativum had percent identity with C. cajan greater than 80% (Tables 2 & 3). Physicochemical properties of matK protein showed that the number of amino acid residues ranged from 199-509 with G.
Why was DNA used as a barcode?
DNA barcoding allows the resolution of taxa from higher (e.g. family) to lower (e.g. species) taxonomic levels, that are otherwise too difficult to identify using traditional morphological methods, like e.g. identification via microscopy.
Why was the rbcL gene used?
The function of the rbcL gene is to code for the large subunit of ribulose 1, 5 bisphosphate carboxylase/oxygenase (RUBISCO or RuBPCase). The sequence data of the rbcL gene are widely used in the reconstruction of phylogenies throughout the seed plants.
What is rbcL marker?
The rbcL marker based method was adopted to identify green isolates from monospecies but non-axenic cultures of microalgae with 18S universal markers as control. In present study, authors have developed and validated a novel method for molecular identification of microalgae even from non- axenic cultures.
Where is MatK gene found?
What is the molecular weight of Maturase K?
The protein runs at approximately 55 kDa, well below the calculated molecular weight of 64 kDa, but in line with previous analyses of MatK (Zoschke et al., 2010). We could not detect MatK in any sample from total leaf protein preparations.