What are the tools used in microarray analysis?
Overview of selected tools
| Tool . | Institution . | Installation successful? . |
|---|---|---|
| ArrayExpress + ExpressionProfiler | EBI, Cambridge, UK | Requires Oracle; analysis not freely available |
| ArrayTrack | FDA, USA | Requires Oracle |
| BASE | Lund University, Sweden | Yes |
| Chipster | Finnish IT Center for Science, Finland | Yes |
How do I check my microarray results?
Quantitative real-time PCR (qPCR) is a commonly used validation tool for confirming gene expression results obtained from microarray analysis; however, microarray and qPCR data often result in disagreement.
Why is PCR used in microarray?
Microarray technology is ideal to screen a lot of genes in one step (>10,000 gene transcripts) and kinetic RT-PCR is very sensitive, highly quantitative and requires up to 1000-fold less RNA. Both allow a relative and accurate quantification of mRNA molecules with a sufficiently high repeatability and low variability.
How much is microarray testing?
These tests are commercially available for $1500-$2000. However, like all medical testing, discounted costs are often arranged between a hospital and reference laboratory or insurance carrier and reference laboratory, which may bring the actual cost of the test quite lower.
What can a microarray not detect?
CMA does not detect: Small changes in the sequence of single genes (point mutations) Tiny duplications and deletions of DNA segments within a single gene (Fragile X syndrome, for example) Balanced chromosomal rearrangements (balanced translocations, inversions)
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