Make sure units match!
How do you find the concentration of antibiotics?
Make sure units match!
- C1V1 = C2V.
- C = concentration; V = volume.
- Example: You have a stock solution of A at 100mg/ml. You want 150 ml at 100ug/ml.
- (100ug/ml)(150ml) = (100000ug/ml)(x)
- 15000 = 100000x.
- x = 0.15ml, or 150ul.
- Remember that you have to convert mg/ml to ug/ml, which you can do by multiplying by 1000.
How much chloramphenicol do I add?
|Antibiotic||Recommended Stock Concentration||Recommended Working Concentration|
|Chloramphenicol||25 mg/mL (dissolve in EtOH)||25 µg/mL|
|Coumermycin||25 mg/mL (dissolve in DMSO)||25 µg/mL|
|Gentamycin||10 mg/mL||10 µg/mL|
|Kanamycin||50 mg/mL||50 µg/mL|
What is the final concentration of the ampicillin in the plates?
Ampicillin is used at a final concentration of 50 µg/ml in both LB-Ampicillin broth and LB-Ampicillin agar. Ampicillin should be stored at –20° and is good for 1 year.
What is the working concentration of chloramphenicol?
Working concentration. 35-50 ug/ml. Chloramphenicol Stock solutions.
How potency is calculated?
The formula provided by CLSI to calculate the potency is shown as: Potency = (Assay purity) * (Active fraction) * (1-Water Content).
How much chloramphenicol do I add to PDA?
You can add chloramphenicol with an average of 0.05 gm. l-1 to PDA medium before autoclaving because the chlramphenicol is not be affected by heating and then put the medium in autoclave at 121 0C and 15 pound. inch -1 for 20-30 minutes.
How much is agar per Litre?
For this purpose 15 grams of agar per liter of distilled or deionized water is the standard recipe.
Why is ampicillin added to LB medium?
It is added to the culture for the best survival of culturing cells during our experiment. If your vector has ampR gene that codes for b-lactamase, then you’d add ampicillin to screen positives. Other reason is, amp is a broad range bacteriostatic antibiotic, which discourages contaminating bacteria from growing.
What is a working concentration?
working concentration is sumthing that is a more dilute form of a stock solution (which is obviously more concentrated) if required volume of stock is too less… there can be two options.. either decrease the stock concentration or increase the working volume!!
How do you make a spectinomycin solution?
Weigh 0.5-1 g of spectinomycin [Spectinomycin /Dihydrochloride Pentahydrate GoldBio Catalog # S- 140, (CAS 21736-83-4, MW = 495.3 g/mol)]. 2. Add 10 ml of sterile H2O. Dissolve completely.
What is the concentration of spectinomycin?
Spectinomycin is a bacteriostatic agent that inhibits translocation of the peptidyl tRNA from the A site to the P site. Up to 100mg/mL stock concentration in H 2 O. 100ug/mL final concentration. Mutations in rpsE, the S5 protein, prevent spectinomycin from binding.
How do you calculate volume from concentration and volume?
Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C 1 V 1 = C 2 V 2. An example of a dilution calculation using the Tocris dilution calculator. What volume of a given 10 mM stock solution is required to make 20ml of a 50 μ M solution?
How do you calculate V1 and V2 from C1 and C2?
Using the equation C 1 V 1 = C 2 V 2, where C 1 =10 mM, C 2 =50 μ M, V 2 =20 ml and V 1 is the unknown: Enter 10 into the Concentration (start) box and select the correct unit (millimolar) Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
How is spectinomycin used to inhibit protein synthesis?
Mutations in rpsE, the S5 protein, prevent spectinomycin from binding. In addition to being used for selection, spectinomycin can also be used to inhibit protein synthesis, for example, to inhibit wild-type ribosome activity in the presence of orthogonal ribosomes. 150-500μg/ml spectinomycin is commonly used for this purpose.