What does KB mean in DNA ladder?
10.0 kilobases
The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb).
How do you make A 1 kb DNA ladder?
If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).
What is A 1 kb DNA size standard ladder?
1 kb DNA ladder consists of 13 linear double-stranded DNA fragments which can be used to determine the size of DNA fragments with 250 bp to 10,000 bp.
What does KB mean in gel electrophoresis?
kilobases
A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb).
How long does DNA ladder last?
1 kb DNA Ladder is stable for at least 3 months at 4°C. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20.
What does KB mean genomics?
A kilobase (kb) is a unit of measurement in molecular biology equal to 1000 base pairs of DNA or RNA.
What does KB mean in genome?
Genome size refers to the amount of DNA contained in a haploid genome expressed either in terms of the number of base pairs, kilobases (1 kb = 1000 bp), or megabases (1 Mb = 1 000 000 bp), or as the mass of DNA in picograms (1 pg = 10−12 g). Genome sizes of bacteriophages and viruses range from about 2 kb to over 1 Mb.
Why is the DNA ladder not separating?
When increasing percentage of low melting agarose gel (1.5% low melting or 1% low melting) the bands and ladder do not separate enough, when running on lower voltage such as 60-80V, for 2-3hours. Increasing voltage would melt the low-melting agarose.
How many KB is the DNA ladder on the Tae gel?
1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/gel lane. The following reagents are supplied with this product:
How many KB plus DNA ladder can be visualized by ethidium bromide?
1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane. The following reagents are supplied with this product:
How much DNA do you put in a DNA ladder?
Mass values are for 0.5 µg/gel lane. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. We recommend loading 0.5 μg of 1 kb DNA Ladder diluted in sample buffer.
What is the recommended gel percentage range for DNA ladder staining?
Recommended gel percentage range: 0.8-1.2% Optimum separation on 1% 1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/gel lane.