What does the SH3 domain do?

The SH3 domain of Src-family PTKs, which regulate many cellular functions, such as cell proliferation and differentiation, survival, migration and cytoskeletal modifications, is mainly involved in substrate recognition and downregulation of the kinase activity.

What is SH3 domain binding?

The SH3 domain is a distinct motif that binds target proteins, including proteins associated with the actin cytoskeleton, through sequences containing proline and hydrophobic amino acids.

What is SH2 and SH3 domains?

SH2 and SH3 domains are small protein modules that mediate protein-protein interactions in signal transduction pathways that are activated by protein tyrosine kinases. SH2 domains bind to short phosphotyrosine-containing sequences in growth factor receptors and other phosphoproteins.

What does SH3 stand for?

The SRC Homology 3 Domain (or SH3 domain) is a small protein domain of about 60 amino acid residues. Initially, SH3 was described as a conserved sequence in the viral adaptor protein v-Crk. This domain is also present in the molecules of phospholipase and several cytoplasmic tyrosine kinases such as Abl and Src.

What role do the Sh2 and SH3 domains play in the function of GRB2?

What role do Sh2 and Sh3 domains play in the function of GRB2? GRB2 is an adaptor protein. It contains one Sh2 domain, and two Sh3 domains on the N terminal and C terminal ends. Sh3 directs the binding of proline rich proteins of other compounds, and Sh2 directs the binding of tyrosine phosphorylated sequences.

Which intracellular proteins contain Sh2 domains?

SH2- or SH3-containing proteins include growth factor receptor–bound protein 2 (GRB2), PLCγ, and the p85 subunit of the phosphatidylinositol-3-kinase.

What do PDZ domains bind to?

PDZ domains bind specific sequence motifs, usually found at the very C-terminus of proteins or, more rarely, at the end of β hairpin structures. PDZ domains bind their ligands, in a manner reminiscent of PTB domains, by incorporating them through hydrogen bonds as an extra strand in a β-sheet.

What is a LIM protein?

LIM domain is a unique double-zinc finger motif found in a variety of proteins such as homeodomain transcription factors, kinases, and adaptors. The LIM-containing proteins are involved in diverse biological processes including cytoskeleton organization, cell lineage specification and organ development.

Is EGFR a tyrosine kinase receptor?

The epidermal growth factor receptor (EGFR) is often considered the “prototypical” receptor tyrosine kinase (RTK) and has been intensively studied. It is one of a family of four RTKs in humans, the others being ErbB2/HER2, ErbB3/HER3, and ErbB4/HER4 (Fig.

Does Ras protein have SH2 domain?

One class of SH2 domain proteins has intrinsic enzymatic activity, e.g., the tyrosine kinase Src, phospholipase Cγ, the tyrosine phosphatase SHP-2, the GTPase-activating protein (GAP) for Ras and the ubiquitin ligase Cbl.

What is a SH3 domain in SRC?

Src Homology 3 (SH3) domains are protein interaction domains that bind proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs. Thus, they are referred to as proline-recognition domains (PRDs).

How do SH3 domains mediate signal transduction?

SH3 domains are protein modules that mediate protein-protein interactions in many eukaryotic signal transduction pathways. The majority of SH3 domains studied thus far act by binding to proline-rich sequences in partner proteins, but a growing number of studies have revealed alternative recognition …

What is the SH3 domain of actin?

The SH3 domain is a distinct motif that binds target proteins, including proteins associated with the actin cytoskeleton, through sequences containing proline and hydrophobic amino acids. A noncanonical SH3 domain-binding motif is positioned between the RCK1 and RCK2 domains of the BK channel α subunit (Fig. 1 ).

What are some examples of proteins with SH3 domain?

Proteins with SH3 domain 1 Signal transducing adaptor proteins 2 CDC24 3 Cdc25 4 PI3 kinase 5 Phospholipase 6 Ras GTPase-activating protein 7 Vav proto-oncogene 8 GRB2 9 p54 S6 kinase 2 (S6K2) 10 SH3D21