What is a LightCycler?

LightCycler technology combines rapid-cycle polymerase chain reaction with real-time fluorescent monitoring and melting curve analysis. Since its introduction in 1997, it is now used in many areas of molecular pathology, including oncology (solid tumors and hematopathology), inherited disease, and infectious disease.

What is Roche Light Cycler?

The LightCycler® 96 Instrument is a real-time PCR system for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping.

What is a PCR platform?

A real-time PCR instrumentation platform consists of a thermal cycler, optics for both fluorescence excitation and emission collection, together with a computer and software for data acquisition and analysis.

What is QuantStudio?

QuantStudio systems enable visualization of your RT-PCR reaction in real-time. The graphical interface simplifies use and provides easy to understand status.

What is the difference between RT-PCR and PCR?

RT–PCR is a variation of PCR, or polymerase chain reaction. The two techniques use the same process except that RT–PCR has an added step of reverse transcription of RNA to DNA, or RT, to allow for amplification.

Is RT-PCR better than PCR?

Real-Time chemistry provides fast, precise and accurate results. Real-Time PCR is designed to collect data as the reaction is proceeding, which is more accurate for DNA and RNA quantitation and does not require laborious post PCR methods.

What is qPCR in LightCycler?

Versatile solutions for gene quantification The LightCycler®480 Software provides multiple solutions for quantitative real-time PCR (qPCR) analyses. Absolute and relative quantification analysis methods and subtypes of these techniques are implemented in the LightCycler®480 Software.

What is the LightCycler® real-time PCR system?

For more than a decade, the LightCycler® Real-Time PCR Systems from Roche Applied Science have stood for flexibility, high speed, and outstanding data accuracy. The LightCycler® 480 System represents the apex of the LightCycler® series, offering a comprehensive, high-end qPCR solution for the modern genomics research lab. 5

What does qRT-PCR stand for?

Real-time, or quantitative, reverse transcription polymerase chain reaction (qRT-PCR), is a powerful method for rapid and reliable quantification of mRNA abundance.

What is the LightCycler 480 instrument?

The LightCycler® 480 Instrument is a high-performance, flexible throughput qPCR platform (with interchangeable 96- or 384-well blocks) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and pathogen detection.