What is hygromycin selection?

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL.

What are selection antibiotics?

There is a long list of antibiotics available to researchers when applying selective pressure in the creation of a stable cell line. The choices include zeocin, hygromycin, blasticidin, puromycin and geneticin (G418).

What is hygromycin A used for?

Hygromycin is an antibiotic that may also be used as an anthelmintic in the form of a feed additive to eliminate or reduce ascarids, nodular worms (Oesophagostomum), and whipworms (Trichuris) of swine, and the large roundworms (Ascaridia) and cecal worms (Heterakis) of poultry.

Are ampicillin and carbenicillin the same?

Carbenicillin differs from ampicillin because it contains a benzyl group as well as a carboxyl group. Remember that this antibiotic has the same mechanism of action as ampicillin since they are both beta-lactam antibiotics.

What is hygromycin B?

Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

Why InvivoGen research reagents?

InvivoGen provides life scientists with innovative research reagents. As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands, cell lines, selective antibiotics, antimicrobial agents, vaccine adjuvants, and antibodies.

Does hygromycin B interfere with translocation?

It has been reported to interfere with translocation [1] and to cause mistranslation at the 70S ribosome [2] . Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species [3-5] .

How many milliliters of hygrogold are in hygromycin?

Hygromycin B Gold (previously named HygroGold™) is supplied as a sterile filtered yellow solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes: ant-hg-1: 10 x 1 ml (1 g) ant-hg-5: 1 x 50 ml (5 g)