What is OT 1 mouse?

OT I Mouse Details These homozygous mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen.

What is OT II mouse?

Common Name: OT-II. These OT-II transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 co-receptor and is specific for chicken ovalbumin 323-339 peptide in the context of I-Ab (CD4 co-receptor interaction with MHC class II).

What are ot i T cells?

These SIINFEKL-specific CD8+ T cells, commonly referred to as ‘OT-I T cells’, have a T cell antigen receptor that consists of α-chain variable region 2 (Vα2) and β-chain variable region 5 (Vβ5), which are inherited via a single transgene1.

Do OT 1 mice have CD4 T cells?

Most dramatically, ≈90% of Vα2+ T cells in OT-1+RAG+TAP−/− mice were CD4+ (Fig. 3B). The OT-1 TCRβ chain has been shown to pair with TCRα chains that differ only in the CDR3 region to generate both CD4+ and CD8+ T cells in mice (36, 37).

What is OVA peptide?

Ovalbumin (OVA) is the major protein in avian egg white, making up approximately 55% of the total. Chicken ovalbumin consists of 385 amino acids, with a relative molecular mass of 42.7 kDa, and has several modifications, including N-terminal acetylation, phosphorylation, and glycosylation.

What is DO11 10?

The DO11. 10 T cell hybridoma is a popular model system used to study CD4+ T cell responses, originally elicited by vaccination of Balb/c mice (H-2d haplotype) with hen egg white ovalbumin (OVA).

What is TCR transgenic mice?

These TCR Tg mice allow researchers to efficiently study T cell-mediated immune responses according to antigen-specific TCR activity by synchronizing all T cell responses to one epitope [4].

Why is ovalbumin used in vaccines?

Ovalbumin (OVA) is a key reference protein for vaccination experiments. Ovalbumin, the major protein constituent of chicken egg whites, is a glycoprotein that is sufficiently large and complex to be mildly immunogenic. Consequently, it is widely used as an antigen for immunization research.

What is Siinfekl?

Ovalbumin peptide SIINFEKL (H-2 Kb) is a single peptide for stimulation of T cells. The peptide from Ovalbumin is synthesized as it is presented by the MHC class I H-2 Kb allele and can be used for targeted in vitro generation and expansion of antigen-specific CD8+ T cells.

What is OT-1 mouse?

Also Known As: OT-1. These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin peptide residues 257-264 (OVA 257-264) in the context of H2K b (CD8 co-receptor interaction with MHC class I).

What are transgenic OT-I mice?

These OT-I mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2K b and used to study the role of peptides in positive selection and the response of CD8 + T cells to antigen. Like most TCR transgenics, these mice are somewhat

What are the transgenic T cell receptors in mice?

These homozygous mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen.

How are Otot-I transgenic mice crossed with C57BL/6NTac targeted mutations?

OT-I transgenic mice were crossed with a homozygous C57BL/6NTac Rag2 targeted mutation model (RAGN12-M), and intercrossed for homozygosity at the Rag2 allele. The model was rederived by embryo transfer and is maintained by mating of male and female mice homozygous for both the Rag2 disruption and the OT-I transgene.