What is Synapsin promoter?
The human synapsin 1 gene promoter targets long-term expression of the EGFP reporter gene exclusively to neurons. A dose of 107 PFU of each vector was injected into either striatum (a–f; n=3 for each vector) or corpus callosum (g–i; n=2 for each vector). In (a) the EGFP fluorescence after Ad. SYN.
What is hSyn promoter?
This biomodule encodes for Human synapsin 1 gene promoter (hSyn). hSyn confers highly neuron-specific long-term transgene expression in the adult rat brain. The AAV collection was developed by Sirion Biotech AG and is made available for research purposes only.
What is SYN1 gene?
SYN1 (Synapsin I) is a Protein Coding gene. Diseases associated with SYN1 include Epilepsy, X-Linked, With Variable Learning Disabilities And Behavior Disorders and Intellectual Developmental Disorder, X-Linked 50. Among its related pathways are Neuroscience and Transmission across Chemical Synapses.
What is ef1a promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
Where is Synapsin expressed?
the brain
Synapsins are evolutionarily conserved phosphoproteins primarily expressed in the brain and fundamental to the regulation of neurotransmitter release.
Where is Synaptophysin located?
It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.
What is EFS promoter?
EF1alpha (or its short, intron-less form, EFS) is a promoter that has been recently used in many clinical trials. It is a cellular-derived enhancer/promoter with decreased cross-activation of nearby promoters, therefore hypothetically decreasing the risk of genotoxicity.
What is synapsin 1 gene promoter used for?
The human synapsin 1 gene promoter targets long-term expression of the EGFP reporter gene exclusively to neurons. A dose of 10 7 PFU of each vector was injected into either striatum (a–f; n=3 for each vector) or corpus callosum (g–i; n=2 for each vector).
Can a fragment of synapsin 1 restrict gene expression to neurons?
In conclusion, we have demonstrated that a short fragment of the human synapsin 1 gene promoter is capable of restricting transgene expression virtually completely to neurons, even in a vector system which efficiently transduces the non-neuronal cells of the brain.
What is the difference between synapsin promoter and NSE promoter?
Although the NSE promoter was relatively neuron specific, it still expressed in glial cells also, and was clearly outperformed by the synapsin promoter with respect to transcriptional neuronal targeting.
What is synapsin family of proteins?
Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases.