What is the purpose of the Ouchterlony test?
The Ouchterlony immunodiffusion assay, developed by the Swedish physician Örjan Ouchterlony, is used for the detection of antigens and antibodies and determination of homologies between antigens (1, 2).
How does the Ouchterlony method help to study the antigen antibody interaction?
In Ouchterlony double diffusion, both antigen and antibody are allowed to diffuse into the gel. This technique can be used to test the similarity between antigens, for example in a study of evolution.
Which medium is used to carried out Ouchterlony double diffusion?
agar
They are radial immunodiffusion (the Mancini method) and double immunodiffusion (the Ouchterlony method); both are carried out in a semisolid medium such as agar.
What is immunoelectrophoresis used for?
The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, especially IgM, IgG, and IgA.
Why is Elisa better than Ouchterlony?
The IND-ELISA results agreed with those from previous numerical taxonomic analyses and Ouchterlony double-diffusion studies. The IND-ELISA method is quicker, more quantitative and less subjective than Ouchterlony assays and thus should be useful in Streptomyces taxonomy.
Which is working principle of ELISA?
Principle of ELISA ELISA works on the principle that specific antibodies bind the target antigen and detect the presence and quantity of antigens binding. In order to increase the sensitivity and precision of the assay, the plate must be coated with antibodies with high affinity.
Why is ELISA better than Ouchterlony?
What is meant by immunoelectrophoresis?
: electrophoretic separation of proteins followed by identification by the formation of precipitates through specific immunologic reactions.
What do you understand by immunoelectrophoresis?
Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are proteins that function as antibodies, which fight infection. There are many types of immunoglobulins that fight different types of infections.
Why study Ouchterlony double diffusion?
To learn the technique of Ouchterlony double diffusion. The key reaction of immunology and immune defense is the interaction of antibodies and antigens. This interaction is responsible for the body’s defense against viral and bacterial infections and other toxins.
What is double diffusion in microbiology?
Double diffusion in two dimensions It is more commonly known as Ouchterlony double diffusion or passive double immunodiffusion. In this method, both the antigen and antibody diffuse independently through agar gel in two dimensions, horizontally and vertically.
What is an Ouchterlony double immunodiffusion plate?
Picture of an Ouchterlony double immunodiffusion plate, after immunodiffusion has taken place. In this, titre value of an antigen is quantified. The central well has an antibody, and the surrounding wells have decreasing concentration of the corresponding antigen.
What is Ouchterlony pattern in immunology?
Ouchtherlony patterns showing partial identity between upper spots. Ouchterlony double immunodiffusion (also known as agar gel immunodiffusion or passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.