Which benchmark cell line verification is recommended by ATCC?

Which benchmark cell line verification is recommended by ATCC?

To standardize STR analysis for human cell line authentication, the American Tissue Culture Collection (ATCC) Standards Development Organization Workgroup has issued standard ASN-0002, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus Amelogenin for …

What is cell strain in cell culture?

A cell strain is a subpopulation of a cell line that has been positively selected from the culture, by cloning or some other method. A cell strain will often have undergone additional genetic changes since the initiation of the parent line.

Are ATCC cell lines authenticated?

Unlike other providers, we authenticate cell lines by comparing the STR profile of sample cell lines with the ATCC Human Cell STR Database.

How long can cell lines be stored at?

Cell lines can be kept at 70C for a few weeks, but they can’t be recovered after 6 months. In a liquid nitrogen freezer, frozen cell lines are stable for a long time.

How do you authenticate a cell line?

The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.

How do I verify cell line identity?

STR-Based Methods Provide Easy, Quick Cell ID Many methods, such as isoenzyme analysis, karyotyping, human lymphocyte antigen (HLA) typing, and characterization of amplified fragment length polymorphisms (AFLP), have been used to identify cross-contamination in cell culture.

What is the difference between cell line and cell strain?

The main difference between cell line and cell strain is that cell line is the first subculture of a cell population of a primary culture whereas cell strain is a subpopulation of a cell line positively selected from the culture after undergoing cloning or some other method.

What happens if cells are over confluent?

When the cells are approximately 80% confluent (80% of surface of flask covered by cell monolayer) they should still be in the log phase of growth and will require sub-culturing. (Do not let cells become over confluent as they will start to die off and may not be recoverable).

Why do you feel necessary to authenticate a cell line?

Cell and microbial authentication is of the utmost importance; skipping the authentication step can lead to lost time and money and retractions of publication as cross-contamination or misidentification has invalidated the data that that research is built upon.

Why do we use ATCC strains?

That’s why ATCC scientists have collected, authenticated, and preserved microorganisms for nearly a century to ensure that researchers have access to the strains needed to develop vaccines and therapeutics, clean up oil spills, ensure healthier food and water, and develop products to protect our health and well-being.